Notes in G2 Introduction to Histology

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Published	11/02/2024	What is the resolution of a human eye? Light microscope? TEM?
Published	11/02/2024	Generally, the ability to {{c1::resolve}} is more limiting than the ability to {{c1::magnify}}
Published	11/02/2024	{{c1::Scanning}} EM permits pseudo-three-dimensional views of the cells and tissues
Published	11/02/2024	In TEM (Transmission Electron Microscopy), what do darker v.s. lighter sections of the image indicate?
Published	11/02/2024	What are the 4 steps in preparing tissues for light microscopy examination?
Published	11/02/2024	Explain the process of Dehydration, Clearing, and Embedding during preparation of tissues for light microscopy:
Published	11/02/2024	How do basic v.s. acidic stains work?
Published	11/02/2024	Hematoxylin- stains {{c1::basophilic}}, negatively-charged (anionic) tissue components- STAINS {{c1::BLUE::color}}
Published	11/02/2024	Eosin- stains {{c1::acidophilic}}, positively-charged (cationic) tissue components - STAINS {{c1::PINK::color}}
Published	11/02/2024	What stain is used for Carbohydrates?
Published	11/02/2024	How does immunocytochemistry staining work?
Published	11/02/2024	What are the 3 steps in preparation of tissues for EM examination?
Published	11/02/2024	{{c1::Chromatin}}, the main component of a cell nucleus is {{c1::basophilic::acidophilic or basophilic}}.
Published	11/02/2024	Euchromatin contains {{c1::genes that are being expressed}}, and are lighter-stained with larger nuclei
Published	11/02/2024	dark and small nuclei with highly condensed chromatin, called {{c1::pyknotic nuclei}} indicate less metabolically active cells.
Published	11/02/2024	Large numbers of ribosomes result in {{c1::cytoplasmic basophilia}}
Published	11/02/2024	In neurons, large basophilic structures comprised of both rER and free ribosomes are known as
Published	11/02/2024	Large numbers of mitochondria result in {{c1::cytoplasmic eosinophilia}}
Published	11/02/2024	Golgi apparatus does not stain with hematoxylin or eosin, therefore in H&E preparations it appears as a clear area close to the nucleus and is ref…
Published	11/02/2024	what dye is used to visualize elastin and elastic fibers
Published	11/02/2024	to visualize reticular fibers -  {{c1::Impregnation with silver salts}}
Published	11/02/2024	what dye is used to visualize collagen
Published	11/02/2024	what is Wright-Giemsa stain used for
Published	11/02/2024	Interpretation of structures in tissue sections:
Published	11/02/2024	Interphase chromatin is present in two forms: more condensed {{c1::heterochromatin}} (stains darker) and less condensed {{c1::euchromatin}} …
Published	11/02/2024	light microscopy (LM) is based on interactions of {{c1::light}} and {{c1::tissue compounds}}electron microscopy (EM) is based on interactions of {{c1:…
Published	11/02/2024	{{c1::Hematoxylin}} and {{c1::eosin (H&E)}} is the most commonly used combination of dyes
Published	11/02/2024	Basophilic structures stain {{c1::blue::color}}Acidophilic structures stain {{c1::pink::color}}
Published	11/02/2024	Artifacts – {{c1::structural abnormalities resulting from the tissue preparation}} (e.g., tissue shrinkage, loss of lipids, loss of mucus)
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