Notes in LE012 DNA Diagnostics: Genomics

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Published	10/04/2024	If a somatic cell divides 5 times, you would expect roughly {{c1:: 5}} mutations
Published	10/04/2024	Sanger Sequencing {{c1:: Isolate a pure DNA fragment in a tube}}Anneal radioactive primers to single stranded DNAAdd polymerase + dNTPs + ddNTP termin…
Published	10/04/2024	Sanger Sequencing Isolate a pure DNA fragment in a tube{{c1:: Anneal radioactive primers to single stranded DNA }}Add polymerase + dNTPs + ddNTP termi…
Published	10/04/2024	Sanger Sequencing Isolate a pure DNA fragment in a tubeAnneal radioactive primers to single stranded DNA {{c1:: Add polymerase + dNTPs + ddNTP termina…
Published	10/04/2024	Sanger Sequencing Isolate a pure DNA fragment in a tubeAnneal radioactive primers to single stranded DNA Add polymerase + dNTPs + ddNTP terminators. D…
Published	10/04/2024	Automated Sanger Sequencing A {{c1:: read}} is the string of contiguous bases {{c2:: generated by one sequencing reaction}}
Published	10/04/2024	Automated Sanger Sequencing The {{c1:: read length}} is the {{c2:: number of contiguous bases generated by one reaction}}
Published	10/04/2024	Automated Sanger Sequencing The {{c1:: error rate}} is the fraction of bases that are {{c2:: read incorrectly by the sequencer}}
Published	10/04/2024	What was the main limitation to first generation sequencing methods?
Published	10/04/2024	Automated Sanger Sequencing The {{c1:: sequencing throughput}} is the number of bases that can be {{c2:: interrogated per unit time/labor}}
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