Notes in 01.1Stains

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Published 07/30/2024 Stains complex carbohydrates a deep-colored red (AKA magenta){{c1::Periodic Acid-Schiff Reactions (PAS)}}
Published 07/30/2024 Mucin produced by goblet cells of the GI and respiratory tract stains magenta with this technique{{c1::Periodic Acid-Schiff Reactions (PAS)}}
Published 07/30/2024 Other PAS-positives:{{c1::Basement membranes and the brush borders of kidney tubules and the small and large intestines CartilageCollagen (to some ext…
Published 07/30/2024 Used to demonstrate supporting tissue elements, principally collagen.{{c1::Masson trichrome}}
Published 07/30/2024 Masson trichrome produces three colors (trichrome): ○ {{c1::Blue}} - nuclei and other basophilic structures ○ {{c1::Green or Blue}} - collagen (depend…
Published 07/30/2024 Useful for blood vessels and skin{{c1::van Gieson}}
Published 07/30/2024 Certain types of mucin, but not all, and cartilage are stained blue{{c1::Alcian Blue}}
Published 07/30/2024 Demonstrates the reticulin fibers of supporting tissue which are stained blue/black{{c1::Reticulin Stain}}
Published 07/30/2024 Traditionally classed as a connective tissue method but is excellent for demonstrating fine cytological detail, especially in epithelium.{{c1::Azan}}
Published 07/30/2024 Standard for staining blood cells and other smears of cells (e.g. bone marrow){{c1::Giemsa}}
Published 07/30/2024 One of the few stains that differentially stain tissues in very thin epoxy resin sections{{c1::Toluidine Blue}}
Published 07/30/2024 This dye also stains mast cell granules reddish purple in paraffin sections, a property called metachromasia.{{c1::Toluidine Blue}}
Published 07/30/2024 Occasionally used today to demonstrate very fine structures such as cell processes{{c1::Silver and Gold Methods}}
Published 07/30/2024 The end product is either black, brown, or golden depending on the method used{{c1::Silver and Gold Methods}}
Published 07/30/2024 distinguish between mineralized bone and unmineralized osteoid{{c1::Goldner’s Trichrome Stain}}
Published 07/30/2024 distinguishes between mineralised bone and osteoid but shows no cellular detail{{c1::von Kossa stain}}
Published 07/30/2024 Uses a basic dye to stain the rough endoplasmic reticulum found in neurons; when this is seen as clumps, it is called Nissl substance.{{c1::Nissl and …
Published 07/30/2024 Stains lipid-containing structures such as myelin a brownish-black colour for light microscopy{{c1::Sudan Black and Osmium}}
Published 07/30/2024 used as the staining agent that provides contrast in electron microscopy{{c1::Osmium}}
Published 07/30/2024 Depend on the exquisite specificity of antibodies for their antigen{{c1::Immunohistological Techniques}}
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