Notes in Laboratory Technology

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Published	10/22/2023	is a technique in which the components of a mixture are separated based on differences in the rates at which they are carried through a stationary pha…
Published	10/22/2023	Formula on getting the number of theoretical plates - Chromatography
Published	10/22/2023	chromatography formula - plate count
Published	10/22/2023
Published	10/22/2023
Published	10/22/2023
Published	10/22/2023	Extraction, 4 main steps in using a separatory funnel
Published	10/22/2023	Fractionating Column - have a high surface area, so are good choice for separation of close-boiling components. However, beads will suffer the greates…
Published	10/22/2023	Fractionating Column - has the least surface area, making it the least capable of separating close-boiling components. Although it has the lowest surf…
Published	10/22/2023	Fractionating Column - have intermediate surface areas and their effectiveness can depend on how tightly the wool is packed in the column. Steel wool …
Published	10/22/2023	Distillation - separating liquids boiling < 150 C
Published	10/22/2023	Distillation - separating liquids boiling >150 C
Published	10/22/2023	Distillation - separating liquid mixtures, soluble in each other, that boil at <25 C from each other.
Published	10/22/2023	Distillation - isolating liquid compounds that are insoluble or slightly soluble in water at all temperatures
Published	10/22/2023	can be used to determine the number of components in a mixture, identify of components, purity of a sample, and can also be used to monitor the progre…
Published	10/22/2023	Thin Layer Chromatography - 3 main steps
Published	10/22/2023	is a technique in which the components of a mixture are separated based on differences in the rates at which they are carried through a stationary pha…
Published	10/22/2023	Naming scheme of chromatography - how chromatographic methods are named
Published	10/22/2023	Most common mobile phases for GC
Published	10/22/2023	Column types depending on ID - Packed with a stationary phase, short column length with large ID, Large amount of sample, Poor resolution
Published	10/22/2023	Column types depending on ID - coated with a stationary phase, long column length with small ID, small amount of sample, better resolution, contain an…
Published	10/22/2023	Detector Advantages - General applicability, simplicity, large linear range, non-destructiveDetector Disadvantages - Low Sensitivity
Published	10/22/2023	Detector Advantages - high sensitivity, large linear range, response is largely indep of flow rate, low noise, ruggedness; ease of useDetector Disadva…
Published	10/22/2023	Detector Advantages - High sensitivity, selective towards X compounds, etc, nondestructiveDetector Disadvantages - Small linear range
Published	10/22/2023	Type of GC
Published	10/22/2023	Type of GC
Published	10/22/2023	part of HPLC - set between the injector and an analytical column, are used to protect analytical columns from impurities in samples. -contains th…
Published	10/22/2023	Mobile Phase in HPLC, types
Published	10/22/2023	HPLC detectors - respond to some property of solutes, such as UV-Vis, absorbance, flourescence, or diffusion current.
Published	10/22/2023	HPLC detectors - respond to a mobile phase bulk property, such as refractive index, dielectric constant, or density
Published	10/22/2023	Normal vs Reverse Chromatography
Published	10/22/2023	type of LC - are based on exchange equilibria between ions in solution and ions of similar charges on the surface of an insoluble, high molecular weig…
Published	10/22/2023	type of LC - is a powerful technique that is particularly applicable to high molecular weight species.
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