Notes in Histotech Ch 1-4 & 6

To Subscribe, use this Key

Status	Last Update	Fields
Published	09/27/2024	Define the purposes of fixation
Published	09/27/2024	Define autolysis
Published	09/27/2024	Define fixation
Published	09/27/2024	Define artifcat
Published	09/27/2024	Define pigment
Published	09/27/2024	Define nonagueous fixative
Published	09/27/2024	Define coagulating fixative
Published	09/27/2024	Define additive fixative
Published	09/27/2024	Define hypertonic
Published	09/27/2024	Define isotonic
Published	09/27/2024	Identify the factors that affect the quality of fixation and describe the effect of each factor on tissue
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ACETIC ACID
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ACETONE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ALCOHOLS
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: B5 FIXATIVE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: BOUINS
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: CARNOY AND METHACARN
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: FORMALIN
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: GENDRE SOLUTION
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: GULTARALDEHYDE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: GLYOXAL
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: HELLY SOLUTION
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: HOLLANDE SOLUTION
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: MERCURIC CHLORIDE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ORTH SOLUTION
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: OSMIUM TETROXIDE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: PARAFORMALDEHYDE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: POTASSIUM DICHROMATE
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ZAMBONI SOLUTION
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ZENKER SOLUTION
Published	09/27/2024	Identify the properties, functions, and actions, and determine whether each action is an advantage or disadvantage: ZINC FORMALIN
Published	09/27/2024	Identify the chemicals in: B5
Published	09/27/2024	Identify the chemicals in: BOUINS
Published	09/27/2024	Identify the chemicals in: CARNOY AND METHACARN SOLUTIONS
Published	09/27/2024	Identify the chemicals in: GENDRE SOLUTION
Published	09/27/2024	Identify the chemicals in: HELLY SOLUTION
Published	09/27/2024	Identify the chemicals in: HOLLANDE SOLUTION
Published	09/27/2024	Identify the chemicals in: ORTH SOLUTION
Published	09/27/2024	Identify the chemicals in: ZAMBONI SOLUTION
Published	09/27/2024	Identify the chemicals in: ZENKER SOLUTION
Published	09/27/2024	Identify which fixatives require postfixation washing, and identify the preferred washing agent
Published	09/27/2024	Identify the fixation pigments and the conditions under which the pigment may be formed
Published	09/27/2024	Identify which of the fixation pigments can be prevented and which of the fixation pigments can be removed: MERCURIC CHLORIDE
Published	09/27/2024	Identify which of the fixation pigments can be prevented and which of the fixation pigments can be removed: POTASSIUM DICHROMATE
Published	09/27/2024	Identify which of the fixation pigments can be prevented and which of the fixation pigments can be removed: FORMALIN SOUTIONS
Published	09/27/2024	State the method(s) of removal; for fixation pigments that can be prevented, state the method(s) of prevention: PIGMENTS, MINERALS, CYTOPLASMIC GRANUL…
Published	09/27/2024	State the method(s) of removal; for fixation pigments that can be prevented, state the method(s) of prevention: MERCURY PIGMENT
Published	09/27/2024	State the method(s) of removal; for fixation pigments that can be prevented, state the method(s) of prevention: LUGOL IODINE SOLUTION
Published	09/27/2024	Explain the difference between buffered and NBF
Published	09/27/2024	State how paraformaldehyde differs from formaldehyde
Published	09/27/2024	Describe the difference between the terms formalin and formaldehyde
Published	09/27/2024	Identify the percentage and volume of formaldehyde in a 1000mL of 10% formalin solution
Published	09/27/2024	Compare and contrast Zenker and Helly fixatives
Published	09/27/2024	List 2 methods of fixation other than using chemical reagents
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: ENZYME HISTOCHEMISTRY
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: IMMUNOFLUROESENCE
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: SKELETAL MUSCLE CROSS-STRIATIONS
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: PHEOCHROMOCYTOMAS
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: EM
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: URATES
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: IHC METHODS
Published	09/27/2024	Identify the preferred method of fixation (or lack of fixation) for: TISSUE FOR TRICHROME STAINS
Published	09/27/2024	Identify which fixative reagents are protein coagulants
Published	09/27/2024	Identify which fixative reagents are non coagulants
Published	09/27/2024	Identify which fixative reagents are additive fixatives
Published	09/27/2024	Identify which fixative reagents are nonadditive
Published	09/27/2024	If the reagent is an additive compound, identify the site or group with which the reagent reacts (if known)
Published	09/27/2024	Describe the effect of acetic acid on erthryocytes and collagen
Published	09/27/2024	Identify any reagents that have associated safety hazards and state the hazard and any special precautions required
Published	09/27/2024	Describe the action of zinc in formalin
Published	10/10/2024	Give the 2 major problems associated with fixation, and ID at least 3 corrective actions for each
Published	09/27/2024	Define dehydration
Published	09/27/2024	Define clearing
Published	09/27/2024	Define infiltatration
Published	09/27/2024	Define embedding
Published	09/27/2024	Define universal solvent
Published	09/27/2024	Define carbowax
Published	09/27/2024	Define cross-section
Published	09/27/2024	Define decalcification
Published	09/27/2024	Define ion exchange
Published	09/27/2024	Define chelatin agents
Published	09/27/2024	Define miscible
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: ETHYL ALCOHOL (dehydrating…
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: METHYL ALCOHOL (dehydratin…
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: BUTYL ALCOHOL (dehydrating…
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: ISOPROPYL ALCOHOL (dehydra…
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: DIOXANE (dehydrating)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: ACETONE (dehydrating)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: TETRAHYDROFURAN (dehydrati…
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: XYLENE (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: TOLUENE (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: BENZENE (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: ACETONE (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: CHLOROFORM (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: CEDARWOOD OIL (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: TETRAHYDROFURAN (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: DIOXANE (clearing)
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: LIMONENE DERIVATIVES (clea…
Published	09/27/2024	List the properties and actions of the following; indicate whether each property or action is an advantage or disadvantage: ALIPHATIC HYDROCARBONS (cl…
Published	09/27/2024	Identify why a concentration of ethanol no higher than 65% should be used after fixation with phosphate-buffered formaldehyde
Published	09/27/2024	State why xylene should be used after clearing with cedarwood oil
Published	09/27/2024	Identify 3 universal solvents
Published	09/27/2024	Compare xylene, limonene derivatives, and the aliphatic hydrocarbons as to physical features, disposal options, and health effects
Published	09/27/2024	State the possible effects on H&E stains if water is present in the clearing agent on the tissue processor
Published	09/27/2024	State how each of the following methods is similar to paraffin and how each differs: CARBOWAX
Published	09/27/2024	State how each of the following methods is similar to paraffin and how each differs: CELLOIDIN
Published	09/27/2024	State how each of the following methods is similar to paraffin and how each differs: GLYCOL METHACRYLATE
Published	09/27/2024	State how each of the following methods is similar to paraffin and how each differs: EPOXY RESIN
Published	09/27/2024	State how each of the following methods is similar to paraffin and how each differs: AGAR AND GELATIN
Published	09/27/2024	State the appropriate processing method or medium for:
Published	09/27/2024	ID the advantages of paraffin compared with other available embedding media
Published	09/27/2024	ID the disadvantages of paraffin compared with other available embedding media
Published	09/27/2024	Relate the melting point of paraffin to hardness, section thickness, and ribboning quality
Published	09/27/2024	ID the problem when: Infiltrating paraffin overheats
Published	09/27/2024	ID the problem when: Embedding paraffin overheats
Published	09/27/2024	ID the problem when: Dehydration is inadequate
Published	09/27/2024	ID the problem when: Dehydration is excessive
Published	09/27/2024	ID the problem when: Clearing is inadequate
Published	09/27/2024	ID the problem when: Clearing is excessive
Published	09/27/2024	Outline paraffin processing schedules
Published	09/27/2024	Describe the microwave processing procedure and ID the differences between the microwave procedure and the procedure used on an enclosed processor
Published	09/27/2024	ID 2 means of decreasing processing time when using paraffin
Published	09/27/2024	ID at least 3 paraffin-processing errors
Published	09/27/2024	Describe the method of correction for: Precipitate in processing chamber and tubing
Published	09/27/2024	Describe the method of correction for: Over-dehydration
Published	09/27/2024	Describe the method of correction for: Poor processing
Published	09/27/2024	ID at least 2 quality control procedures used in processing tissues for paraffin embedding
Published	09/27/2024	Relate rapid chilling of paraffin to crystal size
Published	09/27/2024	State why paraffin wax of small crystalline structure is important
Published	09/27/2024	Describe the proper embedding orientation when embedding: SKIN
Published	09/27/2024	Describe the proper embedding orientation when embedding: TUBULAR STRUCTURES
Published	09/27/2024	Describe the proper embedding orientation when embedding: STRUCTURES WITHIN A WALL (GALLBLADDER)
Published	09/27/2024	Describe the proper embedding orientation when embedding: BONE
Published	09/27/2024	State at least two ways that specimen orientation can be indicated during gross dissection to ensure proper embedding
Published	09/27/2024	Briefly describe the following methods of decalcification: SIMPLE ACID
Published	09/27/2024	Briefly describe the following methods of decalcification: ION-EXCHANGE RESIN
Published	09/27/2024	Briefly describe the following methods of decalcification: ELECTROLUTIC
Published	09/27/2024	Briefly describe the following methods of decalcification: CHELATON
Published	09/27/2024	Describe the methods for determining the endpoint of decalcification
Published	09/27/2024	ID the methods for increasing the rate of the decalcification reaction
Published	09/27/2024	State the effects of overdecalcification on calcified tissue sections
Published	09/27/2024	State the effects of underdecalcification on calcified tissue sections
Published	09/27/2024	State the effects of decalcification on fixed and unfixed tissue
Published	09/27/2024	Describe a method of handling paraffin tissue when areas of calcification are discovered during microtomy
Published	09/27/2024	ID 3 methods of freezing tissue in preparation for cutting frozen sections, and indicate which procedure should be used for skeletal muscle enzymes
Published	09/27/2024	List the equipment in the histo lab that employs temp control and state the proper temp for each instrument
Published	09/27/2024	Describe the following types of microscopy and ID indications for their use: LIGHT MICRO
Published	09/27/2024	Describe the following types of microscopy and ID indications for their use: POLARIZING
Published	09/27/2024	Describe the following types of microscopy and ID indications for their use: PHASE-CONTRAST
Published	09/27/2024	Describe the following types of microscopy and ID indications for their use: DARKFIELD
Published	09/27/2024	Describe the following types of microscopy and ID indications for their use: FLUORESENCE
Published	09/27/2024	Describe the following types of microscopy and ID indications for their use: ELECTRON
Published	09/27/2024	State the most common objectives used in light micro and the approx magnification of each
Published	09/27/2024	Describe the method of determining total magnification
Published	09/27/2024	Define clearance angle
Published	09/27/2024	Define bevel angle
Published	09/27/2024	Define wedge angle
Published	09/27/2024	Define resolution
Published	09/27/2024	Define ocular
Published	09/27/2024	Define objective
Published	09/27/2024	Define micrometry
Published	09/27/2024	Define microtomy
Published	09/27/2024	Define microscopy
Published	09/27/2024	Define achromatic
Published	09/27/2024	Define apochromatic
Published	09/27/2024	Define binocular
Published	09/27/2024	Define parfocal
Published	09/27/2024	List 3 types of microtomes and ID the use of each
Published	09/27/2024	Describe routine microtome maintenance
Published	09/27/2024	ID the diff types of microtome blades
Published	09/27/2024	ID the various blade angles and their significance
Published	09/27/2024	ID at least 6 microtomy problems and the appropriate corrective action for each
Published	09/27/2024	ID at least 4 artifacts that occur during section flotation
Published	09/27/2024	Describe the mechanisms of close tissue-processing systems
Published	09/27/2024	ID critical factors in processing tissue on a short cycle
Published	09/27/2024	Describe the role of vacuum in processing tissue
Published	09/27/2024	Correlate maintenance of all laboratory instruments with quality control and/or quality assurance
Published	09/27/2024	Discuss the principle of pH and the use of a pH meter
Published	09/27/2024	Describe how to obtain an accurate measurement when using a balance
Published	09/27/2024	List at least 3 section adhesives and state why the use of an adhesive may be necessary
Published	09/27/2024	Describe 2 applications in which a microwave oven can be useful
Published	09/27/2024	Describe the principle of microwave-generated heat
Published	09/27/2024	Describe the types of automatic stainers used in histopathology
Published	09/27/2024	Describe the relationship of temperature (blade, chamber, room) to cutting frozen sections
Published	09/27/2024	ID problems encountered when using the cryostat and the appropriate corrective action
Published	09/27/2024	Describe the basic function and benefits of recycling agents
Published	09/27/2024	Describe micropipetting techniques for consistency
Published	09/27/2024	Identify possible chemical, mechanical, and biologic hazards in the histopathology laboratory
Published	09/27/2024	List at least 5 commonly used toxic chemicals and identify the specific hazards of each
Published	09/27/2024	List all reagents used in the lab for fixation and processing of tissue, and ID the hazards assoc with each
Published	09/27/2024	ID the appropriate method of disposal for each of the listed reagents
Published	09/27/2024	List at least 5 flammable or explosive compounds found in the lab
Published	09/27/2024	Discuss proper storage of chemicals that are fire or explosion hazards
Published	09/27/2024	Outline measures to be taken for acid or base spills
Published	09/27/2024	Describe safety measures that should be used because of biological hazards
Published	09/27/2024	List the 4 types of fire extinguishers and ID the type of fire for each would be used
Published	09/27/2024	Define: time-weighted average (TWA)
Published	09/27/2024	Define: Permissible exposure limit (PEL)
Published	09/27/2024	Define: Short-term exposure limit (STEL)
Published	09/27/2024	Define: LD50
Status	Last Update	Fields