Notes in 04EmbeddingSectioningStaining

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04Staining (Group Works)

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Published	07/30/2024	92a5ed82cc784e5bbb3352fc62fe8eb7-oa-1
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Published	07/30/2024	The most important step in Embedding is the correct {{c1::orientation}} of the tissue in a mold. The tissue should be embedded with the surfa…
Published	07/30/2024	In {{c1::embedding}}, the tissue is oriented and placed in a mold that is filled with molten wax to form a solid tissue block
Published	07/30/2024	{{c1::Plastic Mold::Type of Embedding Molds}}- disposable - "peel-away" type
Published	07/30/2024	{{c1::Metal Mold::Type of Embedding Molds}}- not disposable - reusable
Published	07/30/2024	{{c1::MOUNTING}} is the last step in tissue processing. It results in a permanent histological preparation suitable for microscopy, after adhesion …
Published	07/30/2024	{{c1::MOUNTING MEDIUM}} is applied between the section and coverslip after staining and prevents movement of the coverslip.
Published	07/30/2024	The standard method to produce blocks of tissue for section cutting is performed using an {{c1::embedding machine}}, surrounding the tissues b…
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Published	07/30/2024	{{c1::Paraffin Wax}} is the simplest, most common, and best embedding medium used for routine tissue processing
Published	07/30/2024	{{c1::T::T/F}} Paraffin Wax is used in the infiltration step
Published	07/30/2024	Drop 1 or 2 drops of mounting medium::Step 1Place/Position the cover slip over the tissue carefully and correctly to avoid bubbles::Step 2
Published	07/30/2024	{{c1::PERIODIC ACID SCHIFF (PAS) STAIN}} stains {{c2::Magenta/Fuchsia Pink::color}}.
Published	07/30/2024	In {{c1::Sectioning/Microtomy}} a paraffin-embedded tissue is trimmed and cut into uniformly thin slices using a {{c2::microtome}}&nbs…
Published	07/30/2024	In the Step 2 of Sectioning Microtome (STEP 2: ORIENT the block in the microtome chuck ), the long axis of the block should be {{…
Published	07/30/2024	In the Step 3 of Sectioning Microtome (STEP 3: TURN the wheel on the side of the microtome and START CUTTING), the thickness of the bl…
Published	07/30/2024	In the Step 4 of Sectioning Microtome, you continue cutting until you create a {{c1::tissue ribbon}}
Published	07/30/2024	In the Step 5 of Sectioning Microtome (STEP 5: FLOAT the ribbon on the water bath), Water bath temperature should be kept {{c1::5-10}} ̊C {{…
Published	07/30/2024	In the Step 5 of Sectioning Microtome (STEP 5: FLOAT the ribbon on the water bath), the thin sections should look{{c1::Too THIN::Too Thin/T…
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Published	07/30/2024	In STEP 6 of Sectioning Microtome (STEP 6: MOUNT sections on glass slide), the ribbons are removed out of the water bath, put into a glass …
Published	07/30/2024	Good microtomy techniques will minimize {{c1::artifacts}} that can lead to difficult diagnostic interpretation of special stains.  …
Published	07/30/2024	{{c1::Floaters}} are pieces that are not part of the specimen, contaminants
Published	07/30/2024	{{c1::T::T/F}} It is important to regularly change the water in the water bath
Published	07/30/2024	The {{c1::Hematoxylin and Eosin Stain (H&E)::Type of Stain}} is mostly used for routine histopathologic examination
Published	07/30/2024	Eosin Dye - {{c1::acidic::basic/acidic}} - stains cell {{c1::cytoplasm::part of the cell}}, connective tissue, and other extracellular subst…
Published	07/30/2024	{{c1::Hello here's a guide}}
Published	07/30/2024	In Step 2 of Embedding (STEP 2: SELECT the mold that fits the tissue), you have to place the tissue in the middle and have atleast {{c1::2 mm}} of mar…
Published	07/30/2024	In Step 3 of Embedding (STEP 3: DISPENSE wax in the mold), the mold should be about {{c1::1/2}} or {{c1::1/3}} full of wax
Published	07/30/2024	In Step 8 of Embedding (REMOVE the block from the mold when the wax is completely cooled and hardened ), you should wait for around {{c1::30 minu…
Published	07/30/2024	Polysaccharides, particularly glycogen, seen in skeletal and cardiac muscle, liver, and kidneyNeutral mucus substances i.e. glycoproteins, glycolipids…
Published	07/30/2024	Dark blue/Black - Nuclei::Dark blue/BlackRed - Cytoplasm, Muscle, Erythrocytes, Keratin::RedGreen/Light Blue - Collagen::Green/Light Blue
Published	07/30/2024	To differentiate Masson's Trichrome from Oil Red O, remember that 1) Oil Red O is more red and 2) Collagen stains dark blue in Masson's Trichrome w…
Published	07/30/2024	{{c1::ALCIAN BLUE STAIN}} demonstrates {{c2::acid mucopolysaccharides/mucins}}
Published	07/30/2024	{{c1::RETICULIN STAIN}} is a {{c2::black::color}} stain.
Published	07/30/2024	{{c1::Reticular Fibers}} are demonstrated by the use of {{c2::silver stain solutions}} as they are {{c3::argyrophilic}}, which means they ha…
Published	07/30/2024	{{c1::PERL'S PRUSSIAN BLUE}} demonstrates {{c2::iron}} in tissues.
Published	07/30/2024	{{c1::Oil Red O}} and {{c1::Sudan Black}} are notable {{c2::LIPID STAINS::type of stain}}.
Published	07/30/2024	{{c1::Congo Red}} is a {{c2::red-pink::color}} {{c2::amyloid stain::type of stain}} that shows an {{c3::apple-green}} birefringence under polarized li…
Published	07/30/2024	{{c1::GROCOTT-GOMORI METHENAMINE SILVER}} is a {{c2::black::color}} {{c2::broad-spectrum fungal stain::type of stain}}.
Published	07/30/2024	{{c1::GIEMSA STAIN}} stains {{c2::dark blue}} for {{c3::Helicobacter}} and {{c2::purple}} for {{c3::mast cells}}.
Published	07/30/2024	{{c1::GIEMSA STAIN}} is used in staining blood to differentiate {{c2::WBCs}} and for the determination of {{c3::some microbial pathogens (such as&nbsp…
Published	07/30/2024	{{c1::WARTHIN STARRY}} stains organisms {{c2::black::color}} on a background of {{c2::golden yellow::color}} and detects {{c3::spirochetes (Treponema …
Published	07/30/2024	In {{c1::GRAM STAINING}}, a differential staining reaction occurs due to a difference in the permeability, structure, and chemical components of the {…
Published	07/30/2024	{{c1::Gram-positive}} microorganisms stain {{c2::purple::color}} while {{c1::gram-negative}} microorganisms stain {{c2::red::color}}.
Published	07/30/2024	In a {{c1::MYCOBACTERIAL STAIN (ACID FAST STAIN)}}, organisms are stained {{c2::red}} in a sea of {{c2::blue}}.
Published	07/30/2024	In a {{c1::MYCOBACTERIAL STAIN (ACID FAST STAIN)}}, the differential organisms here have {{c2::waxy}} cell walls that can resist {{c3::…
Published	07/30/2024	Open The Tissue CasetteSelect the MoldDispense wax 1/2 or 1/3 FullSelect the tissue and orientInsert the identifying labelDispense wax FullCool the bl…
Published	07/30/2024	Insert the knife blade into the holder and tightenOrient the block in the microtome chuckTurn the wheel on the side of the microtome and start cutting…
Published	07/30/2024	{{c1::Embedding}} is a process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is…
Published	07/30/2024	Parts of the Embedding Machine:{{c1::Wax Dispenser}} - Molten wax is placed here and is dispensed by its mechanism{{c1::Forceps Warmer}} -&nbs…
Published	07/30/2024	Hematoxylin Dye - {{c1::basic::basic/acidic}} - stains {{c1::cell nuclei::part of the cell}} {{c1::purple/blue::color}} - nuclei is the…
Published	07/30/2024	The identification and correct LABELING of tissue blocks and its corresponding slides utilize stickers and barcodes, with the {{c1::second}}…
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