Notes in 13MolecularDiagnosis

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Published	07/30/2024	Prenatal testing is usually performed on cells obtained by {{c1::amniocentesis or chronic villus biopsy::2 methods}}
Published	07/30/2024	Postnatal testing is usually performed on {{c1::peripheral blood lymphocytes::cells}}
Published	07/30/2024	{{c1::Fluorescence In-Situ Hybridization (FISH)::Molecular Diagnosis Method}} - Uses DNA probes + fluorescent dyes that recognize sequences specific t…
Published	07/30/2024	Does the Fluorescence In-Situ Hybridization (FISH) diagnostic method require prior knowledge of the tested abnormality?{{c1::Y::Y/N}}
Published	07/30/2024	{{c1::Array-based Comparative genomic Hybridization (aCGH)::Molecular Diagnosis Method}} - Test DNA and a reference/normal DNA are labeled with 2 diff…
Published	07/30/2024	{{c1::Array-based Comparative genomic Hybridization (aCGH)::Molecular Diagnosis Method}} - Looks for deletions or duplications
Published	07/30/2024	Array-based Comparative genomic Hybridization (aCGH) result skewed towards color of the sample DNA means {{c1::duplication::deletion/duplication}}
Published	07/30/2024	Array-based Comparative genomic Hybridization (aCGH) result skewed towards color of the reference sample means {{c1::deletion::deletion/duplication}}
Published	07/30/2024	Array-based Comparative genomic Hybridization (aCGH) result skewed towards YELLOW means {{c1::none::deletion/duplication}}
Published	07/30/2024	{{c1::Polymerase Chain Reaction (PCR) Analysis::Molecular Diagnosis Method}} - Exponential amplification of DNA or RNA by using appropriate DNA polyme…
Published	07/30/2024	{{c1::Denaturation::Process involved during PCR}} - get two separate standards of DNA
Published	07/30/2024	{{c1::Extension::Process involved during PCR}} - add polymerase for extension of the primer = obtain a new copy of DNA
Published	07/30/2024	{{c1::Sanger Sequencing::DNA PCR Analysis}} - After DNA amplification, DNA polymerase, a DNA primer, nucleotides, and four dead-end (di-deoxy ter…
Published	07/30/2024	{{c1::Allele-specific::DNA PCR Analysis}} - After DNA amplification, fluorescently labeled nucleotides C and T are added which are complemen…
Published	07/30/2024	{{c1::Next Generation Sequencing (NGS)::Molecular Diagnosis Method}} - Solves the downside of Sanger sequencing which looks only at one genetic materi…
Published	07/30/2024	{{c1::Next Generation Sequencing (NGS)::Molecular Diagnosis Method}} - Fluorescently labeled nucleotides are incorporated that are complementary to th…
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