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Notes in 12 Blood Banking, Pre-transfusion Testing, & Hemipheresis

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Published 07/30/2024 Usual Pre-Transfusion Tests done:{{c1::ABO TypingRH(D) TypingCross Matching}}
Published 07/30/2024 RBC carbohydrate antigens develop as early as {{c1::40}} days of fetal life 
Published 07/30/2024 [QC] Frequency1. A2. B3. AB4. O{{c1::O > A > B > AB}}
Published 07/30/2024 The most immunogenic RBC antigens are {{c1::A, B, and RhD::3}}
Published 07/30/2024 Antibodies develop around the {{c1::6}} month of life
Published 07/30/2024 {{c1::Allo}}antibody - Produced in response to an antigen which is not present in the host
Published 07/30/2024 {{c1::Auto}}antibody - React to antigens present in the host cells
Published 07/30/2024 {{c1::Naturally-occuring }}antibody - No need for an immunologic reaction for antibody production
Published 07/30/2024 The HH or Hh gene codes for {{c1::Fucosyltransferase::ABO enzyme}}
Published 07/30/2024 The A gene codes for {{c1::N-acetylgalactosaminyltransferase::ABO enzyme}}
Published 07/30/2024 The B gene codes for {{c1::D-galactosyltransferase::ABO enzyme}}
Published 07/30/2024 Terminal Sugars of {{c2::O}} Blood Group{{c1::Fucose}}
Published 07/30/2024 Terminal Sugars of {{c2::A}} Blood Group{{c1::Fucosyl and N-acetylgalactosaminyl}}
Published 07/30/2024 Terminal Sugars of {{c2::B}} Blood Group{{c1::Fucose and D-galactose}}
Published 07/30/2024 Terminal Sugars of {{c2::AB}} Blood Group{{c1::Fucose and N- acetylgalactosamine and D- galactose}}
Published 07/30/2024 Terminal Sugars of {{c2::Bombay}} Blood Group{{c1::None or N- acetylgalactosamine and/or D- galactose}}
Published 07/30/2024 In the Bombay Blood phenotype, the {{c1::H}} antigen is lacking and it appears as blood type {{c2::O}}
Published 07/30/2024 When Forward Typing a px's blood type, we need...Px RBCsAnti-A Reagent ({{c1::Blue::color}})Anti-B Reagent ({{c1::Yellow::color}})Anti-D Reagent (Rh)
Published 07/30/2024 If the Forward Typing Test agglutinates, then the blood type of the px is {{c1::the same as::the same as/different from}} the antibody
Published 07/30/2024 When forward typing, we use the {{c1::slide}} method at emergencies and bedside
Published 07/30/2024 When forward typing, we use the {{c1::tube}} method at the lab
Published 07/30/2024 What blood Type?Blood Type {{c1::A}}
Published 07/30/2024 What blood Type?Blood Type {{c1::B}}
Published 07/30/2024 Reverse Typing is done only in px {{c1::6 months::age}} and above
Published 07/30/2024 When Reverse Typing a px's blood type, we need...{{c1::Px SerumA RBCsB RBCs}}
Published 07/30/2024 If the Reverse Typing Test agglutinates, then the blood type of the px is {{c1::different from::the same as/different from}} the RBCs added
Published 07/30/2024 Blood type {{c1::O}} is the universal donor for RBCs
Published 07/30/2024 Blood type {{c1::AB}} is the universal donor for plasma
Published 07/30/2024 Blood Type OCan Receive RBCs from: {{c1::O}}Can Receive Plasma from: {{c1::O, A, B, AB}}
Published 07/30/2024 Blood Type ACan Receive RBCs from: {{c1::A, O}}Can Receive Plasma from: {{c1::A, AB}}
Published 07/30/2024 Blood Type BCan Receive RBCs from: {{c1::B, O}}Can Receive Plasma from: {{c1::B, AB}}
Published 07/30/2024 Blood Type ABCan Receive RBCs from: {{c1::O, A, B, AB}}Can Receive Plasma from: {{c1::AB}}
Published 07/30/2024 In Rh Typing, we add Anti-D reagent, centrifuge, and check for agglutination,If (+) Agglutination, Rh{{c1::+}}
Published 07/30/2024 In Rh Typing, we add Anti-D reagent, centrifuge, and check for agglutination,If (-) Agglutination, Rh{{c1::NOT - YET, we test for weak D instead}}
Published 07/30/2024 In Weak D/Du Typing, we add AHG reagent, centrifuge, and check for agglutination,If (+) Agglutination, Rh{{c1::+ even if agglutination is microscopic}…
Published 07/30/2024 In Weak D/Du Typing, we add AHG reagent, centrifuge, and check for agglutination,If (-) Agglutination, Rh{{c1::-}}
Published 07/30/2024 Rh Typing is best done at {{c1::37}} degrees Celsius
Published 07/30/2024 In the lab setup, we perform a {{c1::major}} cross match where we test the Donor {{c1::RBCs}} and Px {{c1::Serum}}
Published 07/30/2024 3 Phases of Cross-Matching:{{c1::Immediate SpinIncubation at 37 Degrees w/ Enrichment MediaAHG Phase or Antiglobulin Crossmatch}}
Published 07/30/2024 Absence or presence of hemolysis indicating compatibility occurs during the {{c1::immediate spin}} phase of cross matching
Published 07/30/2024 During Phase 2: Incubation at 37 degrees w/ enrichment media of Cross-Matching, antigen-ab reactions are boosted with the addition of...{{c1::AlbuminL…
Published 07/30/2024 In cases of emergencies, you can give blood from the {{c1::immediate spin}} phase of cross matching as long as it indicates compatibility
Published 07/30/2024 In antibody screening and identification, it is the same procedure as cross matching, but we add the px serum to {{c1::O RBCs phenotyped w/ different …
Published 07/30/2024 In antibody screening, if agglutination occurs, it is (+) for {{c1::antibody identification}}
Published 07/30/2024 During the Immediate Spin phase of Antibody Screening, below are the possible reactive antibodies:{{c1::Le^a , Le^b M, N Lu^a P1}}
Published 07/30/2024 During the Incubation phase of Antibody Screening, below are the possible reactive antibodies:{{c1::Potent cold (IgM) antibodiesSome warm antibodies, …
Published 07/30/2024 During the Antiglobulin phase of Antibody Screening, below are the possible reactive antibodies:{{c1::Rh antibodies Kell Duffy Kidd S, s Lu^b}}
Published 07/30/2024 Is transfusion reccomended?Cross-matching (-)Ab Screening (+){{c1::Possible. Need to phenotype the donor}}
Published 07/30/2024 Is transfusion recommended?Cross-matching (+)Ab Screening (-){{c1::No}}
Published 07/30/2024 Is transfusion reccomended?Cross-matching (+)Ab Screening (+){{c1::No}}
Published 07/30/2024 Is transfusion recommended?Cross-matching (-)Ab Screening (-){{c1::Yes}}
Published 07/30/2024 There are some instances that we really need to transfuse the blood to the patient. Therefore, we consider giving a {{c1::1}}+ agglutination as it is …
Published 07/30/2024 If you see Auto Anti-I in an Antibody Identification Test, think of... {{c1::Cold Agglutinin SyndromePrimary Atypical Pneumonia::2}}
Published 07/30/2024 If you see Anti-i in an Antibody Identification Test, think of... {{c1::Infectious Mononucleosis}}
Published 07/30/2024 If you see Anti-P1 in an Antibody Identification Test, think of... {{c1::Hydatid Cyst fluid from E. granulosus infection}}
Published 07/30/2024 If you see Auto Anti-P in an Antibody Identification Test, think of... {{c1::Paroxysmal cold hemoglobinuria}}
Published 07/30/2024 If you see Anti-K in an Antibody Identification Test, think of... {{c1::McLeod Phenomenon (+) acanthocytes)}}
Published 07/30/2024 If you see Duffy (Fy a-b-) in an Antibody Identification Test, think of... {{c1::African American population conferring resistance against Plasmodium …
Published 07/30/2024 If you see Kidd in an Antibody Identification Test, think of... {{c1::DHTR}}
Published 07/30/2024 {{c1::Direct Antiglobulin/Coombs Test}} - Detects in vivo sensitization of RBC with IgG and/or complement proteins
Published 07/30/2024 In a Direct Antiglobulin/Coombs Test, we add Add 2 drops of {{c1::polyspecific AHG (anti-IgG + anti complement)}} then check for agglutination
Published 07/30/2024 {{c1::Indirect Antiglobulin/Coombs Test}} - Detects in vitro sensitization of RBC with IgG and/or complement proteins
Published 07/30/2024 Normal Values: Maximum amount of blood that can be taken for hemapheresis{{c1::10.5}} mL/kg of donor’s weight
Published 07/30/2024 2 Types of Apheresis:{{c1::DonationTherapeutic}}
Published 07/30/2024 In what conditions is therapeutic apheresis indicated for...{{c1::Polycythemia vera (usually for phlebotomy)Sickle cell anemiaWaldenström macroglobuli…
Published 07/30/2024 This blood product is prone to bringing sepsis and has the greatest prevalence of bacterial contamination{{c1::Platelets}}
Published 07/30/2024 Platelets are stored at {{c1::25-27}} degrees
Published 07/30/2024 Platelets have a lifespan of {{c1::5}} days
Published 07/30/2024 Normal Values: Donor Plateletpheresis Product Platelet Count{{c1::3.0x 10^11}}
Published 07/30/2024 In donor plateletpheresis products,{{c1::90}}% should be tests under the AABB{{c1::75}}% should be tested under the FDA
Published 07/30/2024 Donor plateletpheresis products should be equal to a pool of {{c1::6}} WB
Published 07/30/2024 Donor Selection for Plateletpheresis:Should not have takenAspirin for {{c1::2}} daysClopidogrel or Ticlopidine for {{c1::14}} daysPlatelet Count >/…
Published 07/30/2024 Donors for Plateletpheresis can only donate...</= {{c1::2}}x per week</= {{c1::24}}% per yearand after {{c1::8}} weeks if w/ WB donation
Published 07/30/2024 Granulocytes are stored at {{c1::20-24}} degrees
Published 07/30/2024 Normal Values: Donor Granulocyte Cytapheresis Product Granulocyte Count{{c1::1.0x 10^10}}
Published 07/30/2024 Donor Selection for Granulocyte Cytapheresis:ABO CompatibilityCMV StatusHLA CompatibilityPlatelet Count >/= to {{c1::150,000}} / UL
Published 07/30/2024 Granulocyte Plasmapheresis also includes:{{c1::CorticosteroidsG-CSF::2 Components/Medications}}
Published 07/30/2024 These conditions are relative contraindications for corticosteroids used for granulocyte mobilization:{{c1::HPNDMPUD::3}}
Published 07/30/2024 These conditions are relative contraindications for G-CSF used for granulocyte mobilization:{{c1::Inflammatory ConditionsGoutRisk Factors for Thrombos…
Published 07/30/2024 Normal Values: Donor Erythrocytapheresis Product Mean Hgb>{{c1::60}} g if packed red cell volume is {{c2::180}} mL>{{c1::50}} g if packed red ce…
Published 07/30/2024 Donor Selection for Erythrocytapheresis:After volume replacement, must not be...Hgb <{{c1::10}} / dLHct must not be <{{c1::30}}%Warfarin should …
Published 07/30/2024 Erythrocytapheresis Donation Deferrals:Single Unit: {{c1::8}} weeksDouble Unit: {{c1::16}} weeks
Published 07/30/2024 Donor Eligibility Following RBC LossInitial RBC loss: <200 mLCombined Initial and 2nd RBC loss within 8 weeks: <200 mLEligibility: {{c1::No Deff…
Published 07/30/2024 Donor Eligibility Following RBC LossInitial RBC loss: <200 mLCombined Initial and 2nd RBC loss within 8 weeks: >200 but </=300 mLEligibility:…
Published 07/30/2024 Donor Eligibility Following RBC LossInitial RBC loss: >200 but </=300 mLCombined Initial and 2nd RBC loss within 8 weeks: N/AEligibility: {{c1::…
Published 07/30/2024 Donor Eligibility Following RBC LossInitial RBC loss: <200 mLCombines Initial and 2nd RBC loss within 8 weeks: >300 mLEligibility: {{c1::Defer f…
Published 07/30/2024 Donor Eligibility Following RBC LossInitial RBC loss: >/=300 mLCombines Initial and 2nd RBC loss within 8 weeks: N/AEligibility: {{c1::Defer for 16…
Published 07/30/2024 Apheresis ADE:{{c1::Citrate ToxicityHypocalcemia}}Vascular ComplicationsVasovagal ReactionHypervolemiaAllergyHemolysisAir embolismClotting Factor Depl…
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