Notes in MGY377 Lec 1 + vid

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Published	09/26/2023	What are the differences between bacteria and viruses?
Published	09/26/2023	Bacteria {{c1::have::have/don't have}} metabolism.
Published	09/26/2023	{{c1::F(many but not all)::T/F}}: All bacteria can dramatically change their morphology.
Published	09/26/2023	{{c1::T::T/F}}: Most eukaryotes on the planet are unicellular (single cells).
Published	09/26/2023	What are the pros and cons from the different growth properties (ex. temp, osmolarity) of different bacteria?
Published	09/26/2023	Bacteria that are difficult to culture are called {{c1::fastidious}}.
Published	09/26/2023	Complex growth media vs chemically defined media
Published	09/26/2023	{{c1::Complex growth media}} contain extracts of plants, yeast or animals (e.g. beef extract). The concentrations of many compounds are not known and …
Published	09/26/2023	{{c1::Chemically defined media}} is made from pure chemicals and the concentration of every chemical in the media is known.
Published	09/26/2023	{{c1::F::T/F}}: The distinction between selective and nonselective media is firm.
Published	09/26/2023	{{c2::LB broth, also called lysogeny broth}}, is one of the most commonly used 'general purpose' bacterial growth media for {{c1::E.coli and related b…
Published	09/26/2023	{{c1::F::T/F}}: LB is nonselective.
Published	09/26/2023	Bacteria grown on LB are mostly eating {{c1::protein}}.
Published	09/26/2023	{{c2::MTGE ('media that grows everything')}} is expensive and difficult to make, and is designed to grow a {{c1::large variety of anaerobic microbes f…
Published	09/26/2023	Common growth media promoters, inhibitors, etc.
Published	09/26/2023	What is agar?
Published	09/26/2023	Agar used for most purposes is a crude extract - composed msotly of {{c1::agaropectin and agarose.}}
Published	09/26/2023	Cheap crude agar used by most labs contains low concentrations of compounds that can feed or inhibit the growth of some species of bacteria. What are …
Published	09/26/2023	What are the advantages of liquid media culturing (rather than solid)?
Published	09/26/2023	What is batch culture and is it common?
Published	09/26/2023	What is continuous culture (chemostat)?
Published	09/26/2023	What is the standard growth curve of bacteria in liquid media in batch culture?
Published	09/26/2023	In what situation would you use solid media?
Published	09/26/2023	What is an indicator/differential media?
Published	09/26/2023	Why is MacConkey Agar described as both selective and differential?
Published	09/26/2023	What is X-gal?
Published	09/26/2023	The final product from beta-galactosidase converting X-gal into galactose and 5-Bromo-whatnot is {{c1::dark blue and precipitates out of solution/does…
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