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MICROBIOLOGY
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Status
Last Update
Fields
Published
09/01/2024
3
Published
09/01/2024
hi
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09/01/2024
What is the definition of microbiology?
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09/01/2024
Lucretius
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09/01/2024
{{c1::Girolamo Fracastoro}} proposed that epidemic disease was caused by transferable tiny particles or "spores"
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09/01/2024
{{c1::image-occlusion:rect:left=.1969:top=.3898:width=.0556:height=.0274:oi=1}}{{c2::image-occlusion:rect:left=.7226:top=.3898:width=.0734:height=.024…
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09/01/2024
What are the 3 different types of domains?
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09/01/2024
Rules of Thumb with Electron Microscopy
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09/01/2024
Compound Light Microscope
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09/01/2024
Types of light microscopy
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09/01/2024
What is the miasmatic theory of disease?
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09/01/2024
Focal Point
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09/01/2024
__________ work established the 3 domains rooted in the comparison of ribosomal RNA genes for microorganisms
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09/01/2024
focal length
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09/01/2024
Hooke coined the term {{c1::cell}} for describing biological organisms
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09/01/2024
Phylogenetic trees with the 3 domains compare how ________ bacteria, eukarya, and archaea are to each other
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09/01/2024
Who was Robert Hooke and what was he known for?
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09/01/2024
{{c1::Anton van Leeuwenhoek}} was the first to observe and describe sincle cells organisms he called animalcules
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09/01/2024
What is spontaneous generation?
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09/01/2024
The {{c1::16S Ribosomal RNA}} helps to determine relatedness between organisms and phylogeny
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09/01/2024
Who were the people to disprove spontaneous generation?
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09/01/2024
Is Bacteria usually single celled or multicelled?
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09/01/2024
strength of lens
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09/01/2024
How did Francesco Redi disprove spontaneous generation?
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09/01/2024
Most bacteria have cells walls with {{c1::peptidoglycan}}
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09/01/2024
Most bacteria {{c1::lack}} a membrane bound nucleus
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09/01/2024
Most bacteria live in {{c1::extreme environments}}
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09/01/2024
What did John Needham claim?
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09/01/2024
There are both {{c1::disease causing}} and {{c1::nondisease causing}} bacteria that exist
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09/01/2024
Who disproved John Needam?
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09/01/2024
Arachae are distinguished from bacteria by {{c1::unique rRNA sequences}}
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09/01/2024
Resolution
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09/01/2024
Many archaea live in {{c1::extreme environments}}
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09/01/2024
Magnification
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09/01/2024
archaea {{c1:: DO NOT}} directly cause disease in humans
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09/01/2024
How did Lazzaro Spallanzani disprove John Neeham?
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09/01/2024
Who was titled 'the high priest of Microbiology'?
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09/01/2024
Numerical Aperture
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09/01/2024
Some archaea have unusual {{c1::metabolic characteristics}}. List a few examples
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09/01/2024
Arachaea have unique {{c1::membrane}} lipids
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09/01/2024
How did Louis Pasteur disprove spontaneous generation?
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09/01/2024
working distance
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09/01/2024
Eukarya {{c1::HAVE}} membrane enclosed nucleus that are larger and morphologically complex
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09/01/2024
What were Pasteur's contributions to microbiology?
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09/01/2024
Who originated the process of pasteurization?
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09/01/2024
Can eukarya be seen without a microscope?
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09/01/2024
What did Pasteur develop vaccines for?
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09/01/2024
{{c1::Protists}} and {{c1::Fungi}} are subcategories of the domain Eukarya
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09/01/2024
Are protists unicellular or multicellular?
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09/01/2024
Oil Immersion
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09/01/2024
Robert Koch provided evidence to cement the {{c1::"germ theory"}} of disease
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09/01/2024
Are Eukarya unicellular or multicellular?
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09/01/2024
What are some examples of Protists and their properties?
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09/01/2024
What are Koch's Postulates?
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09/01/2024
{{c1::Edward Jenner}} used vaccination procedure to protect against smallpox
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09/01/2024
Bright-Field
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09/01/2024
Are fungi unicellular or multicellular?
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09/01/2024
Phase Contrast Microscopy
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09/01/2024
When was life first identified on earth?
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09/01/2024
What are some of the ways we can determine evidence of life on earth?
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09/01/2024
Early earth was hot and {{c1::lacked oxygen}}
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09/01/2024
Cells that were made in early earth must be able to use energy sources under harsh conditions. What were these called?
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09/01/2024
What are stromatolites?
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09/01/2024
The {{c1::oxygen revolution}} caused the extinction of some prokaryotes
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09/01/2024
Who is the founder of modern technology?
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09/01/2024
Others adapted using cellular respiration to harvest energy, this was called {{c1::endosymbiosis}}
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09/01/2024
Define endosymbiosis
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09/01/2024
How was Cock (Koch) revolutionary?
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09/01/2024
What does the susceptipility of an individal depend on?
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09/01/2024
endosymbiosis is the origin of the {{c1::mitochondria}}, {{c1::chloroplasts}}, and {{c1::hydrogenosomes}}
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09/01/2024
What are limitations of Koch's postulates?
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09/01/2024
Koch Postulate 1: The microorganism must be {{c1::present}} in every case of the disease but absent from healthy organisms
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09/01/2024
FUN FACT: Mitochondrial DNA has more homology to any bacteria than our regular DNA in our cells
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09/01/2024
Koch Postulate 2: The suspected microrganisms must be isolated and grown in a {{c1::pure culture}}
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09/01/2024
What does LUCA stand for?
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09/01/2024
Koch Postulate 3: The same disease must result when the isolated microorganism is inoculated into a {{c1::healthy host}}
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09/01/2024
Koch Postulate 4: The same microorganism must be isolated again from the {{c1::diseased host}}
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09/01/2024
What is LUCA?
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09/01/2024
{{c1::Arachaea}} and {{c1::Eukarya}} evolved {{c1::independently}} of Bacteria
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09/01/2024
What are Kock's postulates used for?
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09/01/2024
{{c1::Arachaea}} and {{c1::Eukarya}} diverged from common ancestry
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09/01/2024
Differential Interface Contrast Microscope (DIC)k
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09/01/2024
Eukaryotes increase diversity through {{c1::sexual reproduction}}
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09/01/2024
Bacteria and Archaea increase gene pool by {{c1::horizontal gene transfer}}
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09/01/2024
{{c1::image-occlusion:rect:left=.0642:top=.1913:width=.3301:height=.1028:oi=1}}{{c2::image-occlusion:rect:left=.0687:top=.4039:width=.2941:height=.056…
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09/01/2024
What is taxonomy?
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09/01/2024
what are some ways you can classify living things?
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09/01/2024
What is a strain?
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09/01/2024
Strains can differ in 3 ways, what are the ways?
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09/01/2024
taxonomy of {{c1::macrobes}} are common and easy due to easily distinguishable features and processes. {{c1::Microbes}} are less distinct&nb…
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09/01/2024
How and what for are Dark- Field microscopes used for
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09/01/2024
{{c1::image-occlusion:rect:left=.395:top=.0499:width=.1629:height=.0535:oi=1}}{{c2::image-occlusion:rect:left=.423:top=.1918:width=.1196:height=.0442:…
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09/01/2024
Classification of culture media is based on {{c1::chemical composition}} , {{c1::physical nature}}, {{c1::functions}}
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09/01/2024
Floursecence Microspy- How does UV light work?
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09/01/2024
what does fluorsecence microscopy used for?
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09/01/2024
{{c1::image-occlusion:rect:left=.1511:top=.09:width=.0866:height=.0549:oi=1}}{{c2::image-occlusion:rect:left=.161:top=.2011:width=.0935:height=.0268:o…
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09/01/2024
How does Confocal Microscopy work?
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09/01/2024
Electron Miscroscopy Pros?
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09/01/2024
{{c1::Joseph Lister}} was the pioneer of surgical techniques
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09/01/2024
{{c1::Lister}} developed an {{c1::antiseptic}} surgery system to prevent microorganisms from entering wounds
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09/01/2024
{{c1::lister}} also provided indirect evidence that {{c1::microorganisms}} were the causal agents of disease
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09/01/2024
Who studied lactic acid fermentation that demonstrante the specific cause of milk souring?
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09/01/2024
Why is electron microscopy sooo clear?
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09/01/2024
Transmission Electron Microscope (TEM)
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09/01/2024
Specimen Preparation
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09/01/2024
What was Alexander Fleming best known for?
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09/01/2024
Negative stain
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09/01/2024
The Golden age of microbiology was from {{c1::1850}} to {{c1::1915}}
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09/01/2024
shadowing
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09/01/2024
What is magnification?
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09/01/2024
What is resolution?
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09/01/2024
how does Scanning Electron Micrscope work?
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09/01/2024
What are the steps to gram staining?
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09/01/2024
gram positive bacteria is {{c1::purple}} and gram negative bacteria is {{c1::pink}}
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09/01/2024
Electron Crytography
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09/01/2024
Electron Method w/o Metals? How does it work?
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09/01/2024
What is the advantage of confocal fluorescence microscopy over standard fluorescence microscopy?
Published
09/03/2024
The use of prokaryoe is generally used for which domains?
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09/03/2024
{{c1::Cocci}} are spherical shaped
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09/03/2024
{{c1::Bacilli}} are rod shaped
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09/03/2024
{{c1::Spirilla}} are spiral shaped
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09/03/2024
{{c1::Vibrio}} are slightly curved rods
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09/03/2024
{{c1::Rod}} bacteria are aerodynamic
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09/03/2024
Bacteria are typically {{c1::1-5}} um
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09/03/2024
Large shape or size of bacteria may be {{c1::protective mechanism}} from {{c2::predation and infection}}
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09/03/2024
{{c1::diplococci}} divide and remain in pairs
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09/03/2024
{{c1::streptococci}} are in a singular chain
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09/03/2024
{{c1::staphylococci}} are grape-like in clusters
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09/03/2024
{{c1::Spirochoetes}} are flexible spiral-shaped
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09/03/2024
{{c1::Pleomorphic}} are organisms that are variable in shape
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09/03/2024
What are bacteria with size outliers?
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09/03/2024
Some bacteria grow in more complex multicellular arrangements. List some of these bacteria
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09/03/2024
What is the cell envelope?
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09/03/2024
List the common bacterial features
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09/03/2024
{{c1::Plasma membrane}} is the innermost membrane that encompasses the cytoplasm
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09/03/2024
The plasma membrane is a {{c1::selectively permeable barrier}} that acquires nutrients and eliminates {{c1::waste}}
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09/03/2024
plasma membrane is a thin structure mostly composed of a lipid bilayer that is {{c1::amphipathic}}
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09/03/2024
{{c1::Hopanoids}} are hydrophobic molcules similar to cholesterol
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09/03/2024
Describe ways that the plasma membrane interacts with the external environment:
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09/03/2024
Hopanoids impacts the membranes {{c1::fluidity and shape}} in membrane region
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09/03/2024
Fluidity of the cell is dependent on the {{c1::environment}}
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09/03/2024
The {{c1::cell wall}} maintains the shape of the bacterium
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09/03/2024
The cell wall protects cell from {{c1::osmotic lysis}} and toxic materials
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09/03/2024
{{c1::peptidoglycan}} strands have a helical shape, crosslinked for strength
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09/03/2024
Gram positive bacteria stain {{c1::purple}}
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09/03/2024
Gram negative bacteria stain {{c1::pink}}
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09/03/2024
A {{c1::direct crosslink}} is between carboxyl group and amino groups
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09/03/2024
In an {{c1::indirect cross-link}} a peptide interbridge may form
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09/03/2024
{{c1::Peptidoglycan}} is the rigid mesh-structure lying outside the plasma membrane
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09/03/2024
The alternating sugars of peptidoglycan are {{c1::NAG and NAM}}
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09/03/2024
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09/03/2024
Gram positive cells are composed of {{c1::peptidoglycan}}
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09/03/2024
Gram positive cells walls may also contain {{c1::techoic acid}} which helps to...
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09/03/2024
{{c1::periplasm}} is between plasma membrane and cell wall and has relatively few proteins
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09/03/2024
A lot of bacteria are usually {{c1::negatively}} charged to help with attatchemnt to certain cell types
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09/03/2024
{{c1::gram negative bacteria}} are more complex than gram positive
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09/03/2024
gram negative bacteria consists of {{c1::thin layer}} of peptidoglycan surrounded by an outer membrane made of lipids, lipoproteins, and {{c…
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09/03/2024
Do gram negative cells have techoic acid?
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09/03/2024
The {{c1::lipopolysaccharides}} have a negative charge and help with attatchement in gram negative cells
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09/03/2024
The {{c1::periplasmic space}} constitutes 20-40% of cell volume
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09/03/2024
the {{c1::gram negative}} cell wall contains {{c1::hydrolytic}} enzymes and {{c1::transport}} proteins
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09/03/2024
LPS - lipopolysaccharide provides 3 things, what are they?
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09/03/2024
Capsules are usually composed of {{c1::polysaccarides}}
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09/03/2024
{{c1::Slime layers}} are capsules except dissue, unorganized, and easily removed
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09/03/2024
Describe the protective function of capusles:
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09/03/2024
Slime may facilitate {{c1::motility}}
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09/03/2024
Components outside of cell wall include: {{c1::capusles}}, {{c2::slime layers}}, and {{c3::surface layers (s layers)}}
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09/03/2024
Surface layers have 3 main functions. List these:
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09/03/2024
What is the cytoplasmic matrix composed of?
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09/03/2024
How does the in-folding of plasma membrane to increase surface area benefit the cell?
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09/03/2024
Bacterial cytoskeleton contains protein filaments that...
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09/03/2024
What is intracytoplasmic membrane?
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09/03/2024
Microcompartments
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09/03/2024
{{c1::Gas vacuoles}} are pockets of air that are typically seen in marine bacteria
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09/03/2024
Ribosomes are sites of {{c1::protein synthesis}}
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09/03/2024
What is the size of bacterial and archaeal ribosomes?
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09/03/2024
Bacterial ribosomal RNA(rRNA) are composed of {{c1::16S rRNA}} small subunit and {{c1::23S and 5S}} rRNA in a large subunits
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09/03/2024
The nucleiod is usually {{c1::NOT}} membrane bound and are usually a closed circular DNA
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09/03/2024
Supercoiling and nucleoid proteins aid in {{c1::folding and structure}}
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09/03/2024
{{c1::Plasmids}} are small, closed, circular, independent DNA molcules
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09/03/2024
Plasmids carry {{c1::genes}} that can confer a {{c2::selective advantage}} in some situations
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09/03/2024
{{c1::Fimbriae/Pilli}} are short thin, hair like protein appendeaes to help with movement and attatchment
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09/03/2024
The sex pili is required for {{c1::conjugation}}
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09/03/2024
{{c1::Sex pilli}} are longer thicker, less numerous and required for conjugation
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09/03/2024
The 3 functions of flagella include...
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09/03/2024
{{c1::Flagella}} are threadlike, locomotor appendages extending outward from plasma membrane and cell wall
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09/03/2024
Flagella is composed of 3 parts:
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09/03/2024
{{c1::Filament/Flagellum}} extends from cell surface to the tip
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09/03/2024
{{c1::Hook}} is the short flexible segment (of the flagella)
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09/03/2024
Flagellum is a two part motor producing a torque:
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09/03/2024
{{c1::Rotor}} is a "moving parts", C ring interact with stators and connect to the rod
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09/03/2024
{{c1::Basal body}} is embedded in the cell envelope (part of the flagella)
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09/03/2024
{{c1::Stator}} is the "stationary parts" that campture PMF(proton motor force) through membrane to generate torque and drive rotation
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09/03/2024
{{c1::chemotaxis}} is movement toward a chemical attractant or away from a chemical repellent
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09/03/2024
{{c1::chemoreceptors}} transmit signals throughout the chemosensing system
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09/03/2024
{{c1::image-occlusion:rect:left=.4981:top=.4272:width=.0789:height=.0435:oi=1}}{{c2::image-occlusion:rect:left=.6748:top=.2667:width=.109:height=.0268…
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09/03/2024
presence of attranctant/repellant alters tumbling frequency
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09/03/2024
{{c1::Twitching motility}} are short hairs that move in jerky motions and the cells are in contact with each other and surface
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09/03/2024
{{c1::Gliding}} is smooth movements without appendafes and slime may aid movement on the surface
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09/03/2024
{{c1::Spirochete motility}} are flagella that remain inside the cell wall, winding around the cell and have a corkscrew shape that exhibit flexing and…
Status
Last Update
Fields