Notes in 27 Laboratory Diagnosis of Viruses

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Status	Last Update	Fields
Published	07/30/2024	At which stage/s of viral illness is the virus frequently detectable?{{c1::Onset and Recovery}}
Published	07/30/2024	During the incubation and prodrome period of viral illness, what Ab can be detected?{{c1::Usually None}}
Published	07/30/2024	During the onset of viral illness, what Ab can be detected?{{c1::IgM}}
Published	07/30/2024	During the acute phase of viral illness, what Ab can be detected?{{c1::IgM, sometimes IgG}}
Published	07/30/2024	During the recovery phase of viral illness, what Ab can be detected?{{c1::IgM, IgG}}
Published	07/30/2024	During the convalescence phase of viral illness, what Ab can be detected?{{c1::IgG}}
Published	07/30/2024	4 Types of Diagnostic Methods in Virology:{{c1::Direct ExaminationIndirect Examination or Virus IsolationSerologyMolecular Methods}}
Published	07/30/2024	Latex Agglutination is what category of viral diagnostic method?{{c1::Direct Examination}}
Published	07/30/2024	What do you need to find when looking at a histopath slide of the brain of a px w/ rabies?{{c1::Negri bodies in a purkinje cell}}
Published	07/30/2024	Electron Microscopy is most commonly used in cases of viral {{c1::gastroenteritis}}
Published	07/30/2024	{{c1::10^5 to 10^6::range}} virus particles per mL required for visualization under an E-microscope
Published	07/30/2024	Ideal specimen to use under E-microscopy for HSV and VZV{{c1::Vesicle Fluid}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Papillomavirus{{c1::Skin Scrapings}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Orf{{c1::Skin Scrapings}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Molluscum contagiosum{{c1::Skin Scrapings}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Calcivirus{{c1::Feces}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Astrovirus{{c1::Feces}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Norwalk virus{{c1::Feces}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Adenovirus{{c1::Feces, Nasopharyngeal aspirate}}
Published	07/30/2024	Ideal specimen to use under E-microscopy for Rotavirus{{c1::Feces}}
Published	07/30/2024	What do you need to find when looking at a histopath slide of a px w/ CMV?{{c1::Cytomegalic Viral Inclusions}}
Published	07/30/2024	Ideal specimen to use under Antigen Detection for HCV{{c1::Blood}}
Published	07/30/2024	Ideal specimen to use under Antigen Detection for CMV{{c1::Blood}}
Published	07/30/2024	The influenza virus vaccine is produced and grown in the embryonated egg of a {{c1::hen/chicken}}
Published	07/30/2024	When performing a viral cell culture, these are some of the findings you can use to diagnose:{{c1::CPEHemadsorptionIFInclusion Bodies::4}}
Published	07/30/2024	When performing a viral cell culture on eggs or observing pocks on chorioamniotic membranes, these are some of the findings you can use to diagnose:{{…
Published	07/30/2024	What do you need to find when looking at a cell culture of a px w/ HSV?{{c1::Ballooning and eosinophilic cytoplasm}}
Published	07/30/2024	What do you need to find when looking at a cell culture of a px w/ RSV?{{c1::Syncytia formation}}
Published	07/30/2024	What do you need to find when looking at a cell culture of a px w/ Mumps?{{c1::Syncytia formation & Hemadsorption}}
Published	07/30/2024	When undergoing traditional serology, we take paired sera from 2 phases of the illness:{{c1::Acute PhaseConvalescent Phase (10-14 days)}}
Published	07/30/2024	In primary serology, a {{c1::4}}-fold rise in Ab titer and titrating the serum as {{c1::2}}-fold dilution is taken as a proof of seroconversion
Published	07/30/2024	Can we use Single high titer of IgG or total Ab to determine acute or past infection?{{c1::No. Unreliable}}
Published	07/30/2024	What differences can you observe in the seroconversion of first-time illness and re-infection?First-time: {{c1::Slow decline in IgM and increase …
Published	07/30/2024	[QC] Sharper Rise in Ab titers1. First-time illness2. Re-infection{{c1::B}}
Published	07/30/2024	In a western blot, a (+) test is when we detect at least {{c1::2::n}} types of proteins
Published	07/30/2024	Important proteins associated a western blot and HIV:{{c1::p24, gp41,gp120/160::3}}
Published	07/30/2024	Px w/ {{c1::connective tissue}} diseases will have a false (+) serological result when testing for infectious mononucleosis (EBV)
Published	07/30/2024	Always ask if the px has had {{c1::blood products}} before taking a serological test
Published	07/30/2024	Serology will not be useful in this population{{c1::Immunocompromised}}
Published	07/30/2024	3 Sequential Steps of a PCR:{{c1::DNA Melting or DenaturationAnnealingPrimer Extensions}}
Published	07/30/2024	The DNA Melting or Denaturation Step of a PCR has to work at a temperature of {{c1::95}} degrees
Published	07/30/2024	The Annealing Step of a PCR has to work at a temperature of {{c1::55}} degrees
Published	07/30/2024	The Primer Extension Step of a PCR has to work at a temperature of {{c1::72}} degrees
Published	07/30/2024	A PCR performs {{c1::30-40::range}} cycles
Published	07/30/2024	At what stage of a PCR is DNA used as templates for DNA synthesis and DNA primers attach?{{c1::Annealing}}
Published	07/30/2024	At what stage of a PCR do dNTPs attach to the extending DNA{{c1::Primer Extensions}}
Published	07/30/2024	{{c1::Probe-Based Quantitative}} PCR - Agents capable of generating a fluorescent signal within the PCR are monitored and quantified to reflect the st…
Published	07/30/2024	Composition of the Molecular Beacon Probe in a Probe-Based Quantitative PCR:{{c1::OligonucleotideIntegrated FluorophoreQunecher::3}}
Published	07/30/2024	When you see a px w/ meningitis or encephalitis, where can you collect specimens?{{c1::BloodThroatFecesCSF::4}}
Published	07/30/2024	When you see a px w/ paralytic disease, where can you collect specimens?{{c1::BloodFecesCSF}}
Status	Last Update	Fields