Notes in 10FrozenSection

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Published	07/30/2024	FROZEN SECTION PROCEDUREThe optimal cutting thickness for manual trimming of the block: {{c1::20-40 microns}}
Published	07/30/2024	FROZEN SECTION PROCEDUREThe optimal cutting thickness for sectioning of the block: {{c1::4-7 microns}}
Published	07/30/2024	1. 95% Alcohol (Methanol/Ethanol)2. Water3. Hematoxylin4. Lithium Carbonate or 0.2% Aqueous Ammonia (Bluing)5. Eosin 6. 95% Alcohol7. 100% Alcoho…
Published	07/30/2024	TROUBLESHOOTING{{c1::Ice crystal artifacts}} are due to slow freezing of the tissue
Published	07/30/2024	TROUBLESHOOTINGA {{c1::Knife artifacts}} results rom a nicked cutting blade making a split/tear in the section
Published	07/30/2024	TROUBLESHOOTING{{c1::Over freezing}} can cause the section to have {{c2::holes}}
Published	07/30/2024	TROUBLESHOOTING A solution for Over freezing includes{{c1::Polish block with a couple extra turns of the blade to create friction and warm u…
Published	07/30/2024	TROUBLESHOOTING{{c1::Under freezing}} can be troublesome for {{c2::fatty}} tissue
Published	07/30/2024	TROUBLESHOOTINGSolution for Under freezing includes:{{c1::Add heat sink to block}} {{c1:: or select rapid freeze setting on your cryostat}}
Published	07/30/2024	TROUBLESHOOTINGSolution for Staining Tissues includes:{{c1::Maintain a clean stain line by frequent solution changes}} {{c1::Clean the …
Published	07/30/2024	TROUBLESHOOTINGTo avoid Staining Tissues, water should be changed {{c1::after each frozen section}}, while alcohols and stanges should be ch…
Published	07/30/2024	TROUBLESHOOTING{{c1::Dirty "stain line"}} can cause floaters (extraneous foreign tissue) to adhere to slides
Published	07/30/2024	{{c1::F::T/F}} Stains and alcohols are better diluted to not damage the slides
Published	07/30/2024	TROUBLESHOOTINGSolutions for Air Bubbles include:{{c1::putting an appropriate amount of resin}}{{c1::gently moving air bubbles of slide using fin…
Published	07/30/2024	TROUBLESHOOTING{{c1::Air Bubbles}} may be trapped under cover slips, which can cause the underlying tissue to {{c2::dry out}}
Published	07/30/2024	TROUBLESHOOTING{{c1::Overly thick sections}} may cause the tissue to fall of the slide
Published	07/30/2024	TROUBLESHOOTINGSolution for Overly thick sections includes:{{c1::reducing the cryostat's sectioning thickness}}
Published	07/30/2024	{{c1::Frozen section}} aka {{c1::rush frozen section}} - A laboratory technique used for rapid microscopic and histopathological analysis or…
Published	07/30/2024	3 main indications for a frozen section:{{c1::DiagnosisMarginsAdequacy}}
Published	07/30/2024	[Indication for a frozen section]Identify an unknown pathologic process: whether the lesion is due to an an infection (neutrophil count) or neoplasm (…
Published	07/30/2024	[Indication for a frozen section]Identify metastases{{c1::Diagnosis}}
Published	07/30/2024	[Indication for a frozen section]Simply identify a tissue{{c1::Diagnosis}}
Published	07/30/2024	[Indication for a frozen section]Used to identify the extent of the disease{{c1::Margins}}
Published	07/30/2024	[Indication for a frozen section]Identify that the tissue obtained during biopsy is the lesion of interest{{c1::Adequacy}}
Published	07/30/2024	[Indication for a frozen section]Confirm that pathological tissue is present for diagnosis on permanent sections and what was obtained was not just me…
Published	07/30/2024	Can you perform a frozen section even if there is no immediate need for it?{{c1::N. You don’t request for frozen section if you just want a diagn…
Published	07/30/2024	Can a frozen section act as permanent processing?{{c1::N::Y/N}}
Published	07/30/2024	Specimen should be {{c1::fresh::fresh/fixed}} before frozen section processing
Published	07/30/2024	3 tissue types or conditions that cannot be processed by Frozen Section:{{c1::Bone or other calcified lesionsAdipose tissuesTissues w/ suspected infec…
Published	07/30/2024	Optimum size of a frozen section submission: {{c1::< 8mm x 8 mm x 1-2 mm (thick)}}
Published	07/30/2024	[Frozen Section Materials]Metal platform used for holding embedding medium and tissue for sectioning{{c1::Chuck}}
Published	07/30/2024	[Frozen Section Materials]Embedding medium placed and frozen on chuck to cushion and protect tissue{{c1::Base}}
Published	07/30/2024	[Frozen Section Materials]Tissue frozen on base and covered by additional embedding medium{{c1::Block}}
Published	07/30/2024	The access to the chamber of the cryostat machine is via a {{c1::heated::heated/cooled}} sliding window
Published	07/30/2024	The normal working cryostat machine chamber temperature is from {{c1::0°C to -35::range}}°C
Published	07/30/2024	[Crysostat Machine Component]where the tissue block is placed{{c1::Microtome}}
Published	07/30/2024	[Crysostat Machine Component]where the tissue sections will be obtained after cutting{{c1::Antiroll plate}}
Published	07/30/2024	[Crysostat Machine Component]where the block is placed while waiting for it to solidify{{c1::Tissue shelf}}
Published	07/30/2024	40f6369089ca44618892d60550b0e9ed-ao-1
Published	07/30/2024	40f6369089ca44618892d60550b0e9ed-ao-2
Published	07/30/2024	40f6369089ca44618892d60550b0e9ed-ao-3
Published	07/30/2024	40f6369089ca44618892d60550b0e9ed-ao-4
Published	07/30/2024	Touch preps and smears are often performed on {{c1::suspected malignancies or brain tissue}}
Published	07/30/2024	Are antigens always saved in a frozen section?{{c1::N. They can be altered::Y/N}}
Published	07/30/2024	TROUBLESHOOTINGSolutions for Ice crystal artifacts include:{{c2::Freeze fast (flash/snap)}}{{c2::Smaller Tissues}}{{c2::Avoid Freezing fat around…
Published	07/30/2024	TROUBLESHOOTINGSolution/s for Knife artifacts include:{{c1::Change blade}}
Status	Last Update	Fields