Notes in BIOC-Mini-Exam

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Status	Last Update	Fields
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-1
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-2
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-3
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-4
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-5
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-6
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-7
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-8
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-9
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-10
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-11
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-12
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-13
Published	09/16/2024	d6e82eb036ba49c48be85d09abf2d631-ao-14
Published	09/16/2024	A measure if {{c1::accurate}} if it gives a true {{c2::value}}
Published	09/16/2024	Measurements are {{c1::precise}} if the same measurements can be made again and again
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-1
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-2
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-3
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-4
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-5
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-6
Published	09/16/2024	12b9b45db8e44a05ba072ce844016e1a-ao-7
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-1
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-2
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-3
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-4
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-5
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-6
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-7
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-8
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-9
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-10
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-11
Published	09/16/2024	72a4a0f1ef594bbebcc231c50430af87-ao-12
Published	09/16/2024	A {{c1::solution}} is a chemical (solute) dissolved in a {{c2::solvent}}
Published	09/16/2024	Dilution formula: {{c1::(C1)(V1) = (C2)(V2)}}
Published	09/16/2024	independent variable (ex., time) goes on the {{c1::x axis}} and the dependent variable (ex., absorbance) goes on the {{c1::y axis}}
Published	09/16/2024	Weak acids and bases dissociate to a much {{c1::lesser}} degree than strong acids and bases
Published	09/16/2024	Henderson-Hasselbalch equation.
Published	09/16/2024	{{c1::Buffers}} resist changes in pH because they sop up hydrogen ions or hydroxyl ions
Published	09/16/2024	The pipet must never be held {{c1::sideways}} once it has sample in it
Published	09/16/2024	Beer's Law: {{c1::A = εlc}}
Published	09/16/2024	{{c2::The Bradford Assay}} is an {{c1::indirect}} method because it measures a dye binding to the protein, not the protein itself
Published	09/16/2024	Bradford Assay is measuring {{c1::total protein concentration}} in the sample, not just LDH concentration.
Published	09/16/2024	DNA can be separated and visualized by running {{c1::agarose gelelectrophoresis.}}
Published	09/16/2024	Double stranded DNA can be cut or cleaved by {{c1::restriction endonucleases}} at specific sites.
Published	09/16/2024	{{c1::Activity}}: product formation / time
Published	09/16/2024	{{c1::Enzyme Unit:}} specific amount of product / specific time
Published	09/16/2024	{{c1::Relative Activity:}} units/mL
Published	09/16/2024	{{c1::Unit}}: 1 micromole of pyruvate formed / minute
Published	09/16/2024	{{c1::Specific Activity:}} product form / time/ mL/ amount of protein
Published	09/16/2024	{{c1::Percent Recovery: }}(total units of fraction / total units of crude)100
Published	09/16/2024	{{c1::Fold Purification:}} specific activity of fraction/specific activity of crude
Published	09/16/2024	The father of protein determination is {{c1::Oliver Lowry}}, who invented the {{c1::Lowry}} method.
Published	09/16/2024	Enzymes lower the {{c1::activation energy}} of chemical reactions, thus have a catalytic effect
Published	09/16/2024	{{c1::Centrifugation}} is a method to separate out different components of a cell. It separates the solution into a {{c2::pellet}} and …
Published	09/16/2024	{{c1::Ammonium sulfate}} is highly hydrated and pulls water molecules off of proteins.
Published	09/16/2024	The high salt needs to be removed by {{c1::dialyzing}} the protein preparation.
Published	09/16/2024	The {{c2::dialysis}} membrane has a pore size which retains the {{c1::protein}} but allows the {{c1::salts}} to be remov…
Published	09/16/2024	yout {{c1::mom}}
Published	09/16/2024	{{c1::poo}}
Published	09/16/2024	your {{c1::mom}}
Published	09/16/2024	your {{c1::mom}}
Status	Last Update	Fields