Notes in Lecture 9

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Published	10/19/2024	An {{c2::EM image}} is the 2D projection of a 3D object (plus {{c1::aberrations and noise}})
Published	10/19/2024	{{c2::one}} image is not sufficient to deduce the {{c1::structure}} of a sample particle
Published	10/19/2024	how to get the 3D structure? {{c1::classifying, aligning, and averaging }}
Published	10/19/2024	Cryo images have very low {{c1::S/N}} (low dose to reduce radtion damgae of electron beam + shot noise + aberations)
Published	10/19/2024	Adding {{c1::sine}} waves makes the image more clear and more details
Published	10/19/2024	images (2D or 3D) are made of the sum of {{c1::sine waves}}
Published	10/19/2024	sine waves can be {{c1::equally}} represented in "{{c2::real space}}" or "{{c2::reciprocal space or Fourier space}}"
Published	10/19/2024	imaging processing is computationally {{c1::costly}}
Published	10/19/2024	image {{c1::transformations}} are easier and faster in {{c2::Fourier space }}
Published	10/19/2024	{{c1::FFT}} are also mightly useful in identifiying {{c2::issues with data collection}} in CryoEM
Published	10/19/2024	Real space to FFT conversion is {{c1::lossless}}
Published	10/19/2024	{{c1::Single particle anaylsis}}sample is purified, in solution and mondispersethousands of images are taken at different locationsoritentation determ…
Published	10/19/2024	{{c1::Resolution}} in Cryo is still a "contentious" topic. There are different ways of estimating it
Published	10/19/2024	hard limits of resolution in Cryo{{c1::wavelength of wave}}{{c1::sampling at the camera level }}
Published	10/19/2024	we never {{c1::measure}} the reolution in cryoEM, we {{c1::estimate}} it
Published	10/19/2024	{{c2::decreasing}} angstrom = {{c1::increasing}} resolution
Published	10/19/2024	{{c1::Fourier shell correlation:}} measure of self consistency of the data and the reconstruction process
Published	10/19/2024	fourier shell correlationimages (2D or 3D) are made of the sum of {{c1::sine waves}}sine waves can be equally represented in "{{c1::real space}}" or "…
Published	10/19/2024	{{c2::Cryoelectron tomography }}{{c1::native}}{{c1::hydrated}}{{c1::3D}}
Published	10/19/2024	Cryoelectron tomography con: {{c1::penetration}}
Published	10/19/2024	{{c1::Unleashing the full power of CryoET}}molecular resolution subtomograph averagingcomplementing cellular tomography with corrective imaging&n…
Published	10/19/2024	{{c1::Lamella positioning}} uses the postive cells identified in {{c2::TEM}} to do mill lamella in FIB-SEM
Published	10/19/2024	Integrative imaging goal: {{c1::cover multiple spatial scales }}
Published	10/19/2024	integrative imaging problem: {{c1::resolution vs. field of view }}
Published	10/19/2024	integrative imagining solution: {{c1::combine multiple techniques }}
Published	10/19/2024	integrative imaging{{c1::individual proteins}} AND {{c1::cellular context }}
Published	10/19/2024	CryoEM characteristics{{c1::nativefully hydrated3Dcellular contextmolecular resolutionnon-destructive integration with other techniques}}
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