Review Note

Last Update: 09/23/2023 08:06 PM

Current Deck: Testing Arthur

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how would you solve the issue of having a 'lawn' of bacteria in serial dilution plating?

Back

just count the dilution when there's not too many, not too few colonies

Image

1.0 ml
original culture
(unknown number
1.0 ml
1.0 ml
1.0 ml
1.0 ml
1.0 ml
of cells per ml) So dilution tube #1
Which means
the original
culture flask had
a concentration
of about
740,000 cells
per ml.
had a concentration
of about 74,000
cells per ml.
9.0 ml of
sterile broth
1:10
0.1
Which means dilution
tube #2 had a
concentration of
about 7,400 cells per
ml. (10 times more)
0.1 ml
03
0.1
74
colonies
Remember that we did not
plate 1 ml from these
dilution tubes. We plated
0.1 ml.
So, if there were 74 bacteria
in 0.1 ml that means dilution
tube #3 had approximately
740 bacterial cells per 1
milliliter.
START HERE AND
WORK YOUR
WAY UP...
I would first count this plate. Not too
many colonies...not too few.
6
colonies
colonies
1: 106
0.1 ml
colonies

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