Review Note
Last Update: 10/07/2023 03:20 AM
Current Deck: Testing Arthur::MGY377 Lec 12 (midterm 2)
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Surface display in Gram + bacteria:
- After protein A is made (on ribosome in cytoplasm), it is exported from the cell
- During its export the {{c2::C-terminal hydrophobic and charged domains}} {{c1::temporarily restrain the protein in the membrane::do what}} →
- → {{c3::sortase (membrane bound enzyme)}} recognizes the {{c4::LPXTG motif of protein A and cleaves the signal (between T and G residues)}} → {{c5::get energy (no need ATP?) }}
- → {{c6::newly formed carboxy terminus of protein A is attached to sortase enzyme }}
- The removed C-terminal sorting sequence ({{c8::hydrophobic}} domain and charged {{c8::glycine}} tail) is {{c7::degraded }}
- → sortase enzyme {{c9::links the new C-terminus of protein A (LPXT) to a lipid linked cell wall subunit }}
- → peptidoglycan precursor linked to protein A is incorporated into net of cell wall by {{c10::transpeptidation and transglycosylation reactions}}. Protein is now attached to cell wall and{{c11::the lipid carrier is recycled for another round of cell wall synthesis or protein anchoring::what happens for the next round of cell wall synthesis to occur}}
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