Notes in BIOC 4521

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Status	Last Update	Fields
Removal Requested	10/19/2024	hguh {{c1::hi}}
Published	10/19/2024	{{c1::Xray diffractometry, crystallography, XRC:}}• The {{c2::oldest}} of the methods• Continues to contribute the most depositions• Can do *{{c2::Som…
Published	10/19/2024	{{c1::Nuclear magnetic resonance spectroscopy, NMR:}}• {{c2::Small to medium}}-sized structures (size-to-difficulty: {{c2::exponential}})• Fantastic a…
Published	10/19/2024	{{c1::Cryo-electron microscopy, CEM:}}• {{c2::medium to huge}} structures (size-to-difficulty: {{c2::linear}})• Not good for {{c2::dynamics}}• Not dep…
Published	10/19/2024	{{c1::Computational (QM & MM):}}• {{c2::Small to medium-large}}-sized structures (size-to-difficulty: {{c2::exponential}})• Can calculate {{c2::dy…
Published	10/19/2024	Structure size keysmall: {{c1::0-200 AA}}medium: {{c1::200-1,000 AA}}large: {{c1::1,000+ AA}}
Published	10/19/2024	XRC diffraction spots are in {{c1::reciprocal space}}
Published	10/19/2024	XRC diffraction spots are the result of {{c1::complex wave interactions}} from all atoms in the crystal
Published	10/19/2024	{{c1::macromolecules}} in a {{c1::crystal lattice}} are far more complex than a sphere of mirrors
Published	10/19/2024	Macromoleulces have light "reflecting" planes of matter called {{c1::Bragg planes}}
Published	10/19/2024	The spots from {{c1::crystal matter planes}} can be used to back calculate {{c2::electron density}} that causes the spots
Published	10/19/2024	If we know {{c2::electro density}}, we have the {{c1::structural model }}
Published	10/19/2024	hi {{c1::timing-leopard-india-pennsylvania-oregon-virginia}}
Published	10/19/2024	Enzymes in crystals are generally still {{c1::active}}, but their {{c1::kinetics}} are slowed
Published	10/19/2024	We are able to determine {{c2::dynamics/mechanism}} from {{c1::XRC}}
Published	10/19/2024	For NMR, need an {{c2::odd}} number of {{c1::nucleons}}
Published	10/19/2024	{{c1::Nucleon}}: proton or nuetron
Published	10/19/2024	Need to feed organism an odd number like {{c1::carbon-13::carbon what}} to see on NMR
Published	10/19/2024	Precession is always {{c1::constant}}
New Card	10/15/2024	hello this is {{c1::your mom}}
Removal Requested	10/19/2024	your mom {{c1::meeps}}
Published	10/19/2024	{{c1::Perturbation}} causes the precession to wobble and in the case of nuclear spin, even {{c2::invert}}
Published	10/19/2024	Plotted in 2D, this is called the {{c1::free induction decay (FID)}}
Published	10/19/2024	The {{c1::fourier transform}} of the FID converts the specttrum from the {{c2::time}} domain to the {{c2::frequency}} domain, and yields {{c3::intepre…
Published	10/19/2024	Fourier transform is a form of calculus that {{c1::inverts the units}}
Published	10/19/2024	Signal to noise improvement through {{c1::wave addition }}
Published	10/19/2024	Noise is {{c1::random}} and therefore {{c2::cancels}}, signal is {{c1::consistent}} and therefore {{c2::additive}}
Published	10/19/2024	Computational models are getting more and more {{c1::reliable}}
Published	10/19/2024	Strengths of XRC: Can take as little as {{c1::2-3}} weeks Very high {{c1::resolution}} structures are possible {{c1::De novo}} method&n…
Published	10/19/2024	Weaknesses of XRC: Takes a lot of training {{c1::Insoluble}} proteins are hard to crystallize {{c1::Dynamics}} not always possible&nbsp…
Published	10/19/2024	The crystal is used as an {{c1::amplification}} device.
Published	10/19/2024	Much of the XRC data is {{c1::occluded}}.
Published	10/19/2024	Macromolecular crystal growth is a slow, controlled {{c1::precipitation}} under {{c1::aqueous}} conditions that do not denature proteins.
Published	10/19/2024	{{c1::Precipitants}} for crystal creation are {{c2::ionic salts}} and {{c2::organic solvents}} like PEGs
Published	10/19/2024	{{c1::Vapor diffusion}} is a popular setup for protein crystallization
Published	10/19/2024	For any given positioning of a crystal in the x-ray beam, many {{c1::Bragg planes}} are reflecting beams. This is why any given 2D image has many spot…
Published	10/19/2024	{{c1::Bragg's Law}}: x-rays diffract around planes of matter in the crystal.
Published	10/19/2024	Bragg’s Law can be explained in terms of trigonometry and {{c1::wave interference (destructive and constructive)}}. nλ = {{c2::2d(sinθ)}}
Published	10/19/2024	The single object defines a continuous background heat map. The {{c1::crystal lattice}} imposes the “spots” through which the continuous background he…
Status	Last Update	Fields